N15 Cro and λ Cro: Orthologous DNA-binding domains with completely different but equally effective homodimer interfaces

Structural similarity in the DNA-binding domains of catabolite gene activator and cro repressor proteins.

It is shown that there is a structural similarity between the presumed DNA-binding regions of the Escherichia coli catabolite gene activator protein ("CAP") and the cro repressor protein ("cro") from bacteriophage lambda. The correspondence between the two proteins is particularly striking for a structural unit consisting of two consecutive alpha-helices. The 24 alpha-carbon atoms that constitu...

Different interactions used by Cro repressor in specific and nonspecific DNA binding.

The mode of interaction of Cro repressor with specific and nonspecific sites on DNA was explored by chemical modification and protection of lysine and tyrosine residues. Cro has 8 lysines. In the presence of DNA, lysines 32 and 56 are fully protected and lysines 21, 62, and 63 are partially protected from alkylation. However, the terminal amino group and lysines 8, 18, and 39 are not protected....

Contract Research Activity (cro)

Cro repressors from engineered mutagenesis of a synthetic cro gene ( DNA synthesis / synthetic gene / protein - DNA interactions )

A portion of the gene coding for the Cro repressor protein of bacteriophage X has been chemically synthesized, incorporating base pair changes that generate restriction endonuclease sites without altering the amino acid coding sequence. These restriction endonuclease sites were used to remove small segments of the synthetic cro gene and the segments were replaced with duplexes carrying desired ...

Ion Implanter Cro Safety Considera

The contamination on previous been previously reported with emphasis o investigations done earlier but with some Arsenic in beamlines, target chambers an TLV locally for extended periods during source chambers in situ or on a bench top of several hundred ppb or more. Freshly c and AlP and AlAs in quantities that need changes but that use is not always dictate under a designated, remote work are...